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KMID : 1033620120390020073
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Clinical and Experimental Reproductive Medicine
2012 Volume.39 No. 2 p.73 ~ p.80
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Effects of hypoxia inducible factors-1α on autophagy and invasion of trophoblasts
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Choi Jong-Ho
Lee Hyun-Jung
Yang Tae-Hyun
Kim Gi-Jin
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Abstract
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Objective: This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-1α on the cell death, autophagy, and invasion of trophoblasts.
Methods: To understand the effect of HIF-1α, we inhibited HIF-1α using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-1α. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy.
Results: There was no difference in cell death through the inhibition of HIF-1α expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-1α expression by siRNA. These experimental results mean that HIF-1α genes regulate the invasive ability of trophoblasts by increasing autophagy.
Conclusion: This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-1α and autophagy.
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KEYWORD
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Placenta, Trophoblasts, Hypoxia, Invasiveness, Programmed cell death type II
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